Only a few reports [ 4, 5, 10, 11, 12, 13] describe solvent-free MALDI-MS for the analysis of proteins and peptides. In this study, we developed a universal mini-ball I. Solvent-free based on the mini-ball mill (MBM) method. A general solvent-free protocol was used [4]. Mixing of the analyte and matrix powder was performed using Solvent-free MALDI-MS for the Analysis of β-Amyloid
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A mini ball mill (MBM) solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) method allows for the analysis of A mini ball mill (MBM) solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) method allows for the analysis of Solvent-free MALDI-MS for the analysis of a membrane
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Manual and automated solvent-free mini-ball mill (MBM) matrix-assisted laser desorption/ionization (MALDI) analysis of mixtures of beta-amyloid peptides (1-11), (33 The method and clean-up issues related to solvent-free MALDI-MS, which were considered a serious obstacle for routine and high throughput use, can be Solvent-Free MALDI-MS: Developmental Improvements in
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Semantic Scholar extracted view of "Solvent-free MALDI-MS for the analysis of β-amyloid peptides via the mini-ball mill approach: Qualitative and quantitative advances" by S. An efficient, low sample load mini-ball mill (MBM) sample preparation procedure was developed for solvent-free MALDI analysis of peptides and proteins. Picomole sample Solvent-free MALDI-MS for the analysis of biological samples via a mini
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Abstract. A mini ball mill (MBM) solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) method allows for the analysis of Solvent-free MALDI simplifies the sample preparation by removing the influence of solvent and provides, in cases where the solvent is problematic, Multisample Preparation Methods for the Solvent-free MALDI
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Manual and automated solvent-free mini-ball mill (MBM) matrix-assisted laser desorption/ionization (MALDI) analysis of mixtures of beta-amyloid peptides (1-11), (33-42), (1-42) and non-beta-amyloid component of Alzheimer's disease peptide yielded interpretable spectra for all of the peptides present regardless of their relative amounts in the samples.A limitation of any current approach using solvent-free MALDI mass spectrometry is that only one sample at a time can be prepared and transferred to the MALDI-plate. For this reason, multiple-sample preparation approaches for solvent-free MALDI MS analysis of synthetic polymers were developed that are simple and practical. Multisample preparation methods for the solvent-free
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vent is not an issue, the solvent-free method is generally less efficient. Workflow and clean-up issues related to solvent-free MALDI analysis were considered serious handicaps for high-throughput applications. These res-ervations are partially overcome by the vortex [6] and mini-ball-mill (MBM) methods [7–9]. An on-targetManual and automated solvent-free mini-ball mill (MBM) matrix-assisted laser desorption/ionization (MALDI) analysis of mixtures of β-amyloid peptides (1–11), (33–42), (1–42) and non-β-amyloid component of Alzheimer’s disease peptide yielded interpretable spectra for all of the peptides present regardless of their relative amounts in Solvent-free MALDI-MS for the analysis of Springer
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In this paper, the influencing factors of the synthesis of MOFs by ball milling are systematically reviewed ( Figure 1 ), including the effect of auxiliary additives, metal source type, ligand type, and reaction conditions, and the future outlook of development will be discussed. Figure 1. Scheme of the synthesis of metal organic frameworks byA mini ball mill (MBM) solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) method allows for the analysis of bacteriorhodopsin (BR), an integral membraneSolvent-Free MALDI-MS for the Analysis of a Membrane
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A mini ball mill (MBM) solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) method allows for the analysis of bacteriorhodopsin (BR), an integral membraneA mini ball mill (MBM) solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) method allows for the analysis of bacteriorhodopsin (BR), an integral membrane protein that previously presented special analytical problems. For well-defined signals in the molecular ion region of the analytes, a desalting procedureSolvent-Free MALDI-MS for the Analysis of a Membrane
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Solvent-Free MALDI-MS Based on the Mini-Ball Mill (MBM) Method A general protocol was developed by adapting existing grinding procedures [5, 8] in order to downscale the method for biologically-relevant analyte amounts (Ta-ble 1). The sample/matrix-ratio was varied, as were other experimental conditions which are Manual and automated solvent-free mini-ball mill (MBM) matrix-assisted laser desorption/ ionization (MALDI) analysis of mixtures of -amyloid peptides (1-11), (33-42), (1-42) and non- -amyloid component of Alzheimer’s disease peptide yielded interpretable spectra for all of the peptides present regardless of their relative amounts in Solvent-free MALDI-MS for the analysis of <Emphasis
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A mini ball mill (MBM) solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) method allows for the analysis of bacteriorhodopsin (BR), an integral membrane protein that previously presented special analytical problems. For well-defined signals in the molecular ion r In ball mill mixers, a jar (sometimes called vessel or bowl) is charged with milling or grinding media (also known as balls or beads) that are moved and mixed with the raw materials and reagents. water) or eliminate their use to achieve significant system cost reduction. Therefore, solvent-free processing methods would represent aMixing methods for solid state electrodes: Techniques,
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High‐speed ball‐milling: Direct mechanocatalysis is a new catalysis concept using high speed ball milling conditions. The surface of the milling balls is the catalytic active site, hence the recovery of the catalyst is convenient and efficient. Being a solid state technique, solvent related problems are omitted.You can not select more than 25 topics Topics must start with a letter or number, can include dashes ('-') and can be up to 35 characters long.sbm/sbm ball ball milling lecture in mini projrct.md at
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We report here an understanding of systems chemistry on small molecules through covalent mechanochemistry. As a proof-of-concept, the multicomponent Biginelli reaction by subcomponent synthesis was considered as a model system. Reactions were performed under solvent-free, metal-free, mechanochemical (ball milling) and ambient Ball mill is an effective, and green method for the synthesis of heterocyclic compounds in very good yields. This method is a simple, economical, and environmentally friendly process. [2,3-c]pyrazole derivatives under ball milling and solvent-free conditions. Unlike other pyranopyrazoles synthesis reactions, this method has advantagesA green protocol ball milling synthesis of dihydropyrano [2,3-
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Manual and automated solvent-free mini-ball mill (MBM) matrix-assisted laser desorption/ ionization (MALDI) analysis of mixtures of -amyloid peptides (1-11), (33-42), (1-42) and non- -amyloid component of Alzheimer’s disease peptide yielded interpretable spectra for all of the peptides present regardless of their relative amounts in An efficient, low sample load mini-ball mill (MBM) sample preparation procedure was developed for solvent-free MALDI analysis of peptides and proteins. Picomole sample amounts can be handled conveniently, with 30 s grinding times being sufficient. Matrix purity and molar analyte/matrix ratios are not as critical as with methods Solvent-free MALDI-MS for the analysis of biological samples via a mini
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The activation of zero-valent metals using ball milling does not require pre-dried and deoxygenated solvents, nor is a blanket of nitrogen or argon gas necessarily required; further, mills can beAbstract. An expedient, mechanochemical, operationally simple protocol is reported for the Rh-catalysed C–H methylation of (hetero)arenes under solvent-free conditions without the use of a ball mill. Reagent mixing and activation are delivered using simple pestle-and-mortar grinding and subsequent heating, providing access to the sameSolvent-free and ball mill-free catalytic C–H methylation
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